Biol 130 First Midterm Notes

Unit 1 Introduction to the Cell Robert Hooke built the starting line microscope (30x magnification) viewed slices of phellem c anyed cadreula (little rooms). Antoni Van Leeuwenhoek worked with glass huge improvement in quality of lenses nearly 300x magnification became possible first to ob dish * iodine- jail cellular ph nonp ariled organisms animalcules * protists from pond body of weewee * b doingerium from his m step forwardh father of microbiology * blood cells * banded frame in muscle cells * sperm from 1830s Compound microscope improved magnification and resolution and in allowed visualization of objects less than 1 ? . 1000-1500x magnification Beginning of Cell system Robert Brown (botanist) noticed that either plant cell contained a round structure called it kernel-nucleus Matthias Schleiden (an other(a) botanist) all plant tissues be composed of cells embryonic plant endlessly arose from a single cell Theodor Schwann (zoologist) similar observation s in animal cells recognition of structural similarities btw plants and animals * Cell Theory approach patternulated by Schwann Cell Theory 1. all organisms consist of one or much cells 2. he cell is the basic unit of structure for all organisms 3. added 20 years later all cells spring notwithstanding from pre-existing cells fact (scientific) an attempt to state our best current on a lower floorstanding, ascendantd on observations and experiments(valid only until revised or replaced) Steps in Scientific Method 1. make observations 2. use inductive cerebrate to develop tentative explanation (hypothesis) 3. make predictions basebornd on your hypothesis 4. make further observations or design and carry out controlled experiments to test your hypothesis 5. nterpret your results to see if they support your hypothesis Theory a hypothesis that has been tested critically under many variant conditions andby many different investigators . using a variety of different approaches. By the time an explanation is regarded as a possibility it is widely reliable by most scientists in the cell * the solid ground of science evolution, germ theory, cell theory *If a theory is thoroughly tested and confirmed over many years by much(prenominal) large numbers of investigators that there is no doubt of its validity it may eventually be regarded as a law.Gravity, laws of thermodynamics, laws that govern behaviour of gases Strands of Cell Biology 13 cytology 1600s Hooke looks at cork Leeuwenhoek looks at lashings of things 1800s Brown notes nuclei bio-chemistry synthesis of urea in lab fermentation done by cells glycolysis Krebs cycle e very(prenominal) cell comes from a cell Schleiden & Schwann act uponulate cell theory electron microscopy stains & dyes transmissibles Mendel, pea plants deoxyribonucleic acid chromosomes chromosome theory 1930s desoxyribonucleic acid manifold helix desoxyribonucleic acid sequencing Dolly the sheep nano-technology communicable code blithesome MicroscopyBright bailiwick light passes through specimen, contrast is slow and specimen is hard to see stagecoach contrast contrast is changed by changing light in microscope DIC uses optical modifications to change contrast betwixt cell and background due to density differential Staining stain used to visualize cell and components, only some stains give the axe be used on living cells 14 bright field phase contrast DIC unstained (sperm cells) stained blood cells tissue small intestine Fluorescent Microscopy fluorescent fixture dyes bind to protein or DNA to see where they are in cells tracks movement Electron Microscopy(S postning & Transmission)SEM scan rise of specimen to form image by detecting electrons from outer surface. Good surface images TEM forms image from electrons dismission through specimen therefore fine details of internal organelles 16 SEM TEM Basic Properties of Cells * are exceedingly complex and organized * components molecules macro molecules (organelles ) enclosed in plasma tissue layer * use the identical genetic platform Central Dogma * DNA RNA protein * are capable of reproducing themselves * must first replicate genetic material acquire and use might (bioenergetics) and carry out a variety of chemical chemical reactions (cellular metabolism) * guide many processes that are highly conserved at the molecular level * membrane structure, genetic code, adenosine triphosphate synthesizing enzymes, actin filaments, eukaryotic flagella, * engage in many mechanical activities * transport of materials in/out, indoors * assembly and disassembly of structures * motility / movement * oppose to environmental signals * move away or toward stimuli * respond to hormones, growth factors, etc * are capable of self-regulationhomeostasis most evident when control systems break down defects in DNA replication, DNA repair, cell cycle control Two Classes of Cells karyon = nucleus Prokaryotic Cells lack of nucleus, NO CY TOSKELETON(very small), membrane bound organelles. intimatelyly unicellular. bacterium and Archaea. Single, circular strand of DNA(fewer proteins). Cell groyne in appendix to PM 1-10 uM in diameter. 2 compositors cases 1. Eubacteria all keep up cells walls except for mycoplasma(resistant to antibiotics that target cell wall synthesis). Mycoplasma(smallest) Cyanobacteria (largest and most complex). 2.Archaeabacteria all confound cell walls and are k todayn as extermophiles, occupy massive range of habitats, halophiles=salty, acidophiles=acid, thermophiles= hot. Eukaryotic Cells 10x larger than prokaryotic cells, membrane bound nucleus/organelles. More complex DNA due to histones/proteins. 4 free radicals 1. Protists- very diverse group mostly single cells algae, piss molds, slime molds, phylum Protozoa 2. Fungi single cell(yeast) or multi-cellular(mushrooms) and have cell walls. Heterotrophs depend on external source of original compounds 3. Plant cells- multi-cellular and have cell walls. . Animals- multi-cellular, no cell walls and are heterotrophs Cytoplasm everything mingled with plasma membrane and nuclear membrane, includes all membrane-bound organelles (except nucleus) Cytosol only fluid component Endomembrane system internal membranes that are either in direct contact or connected via transfer of vesicles (sacs of membrane). including nuclear envelope / membrane, endoplasmic reticulum (ER), Golgi apparatus, lysosomes, vacuoles Nucleus stores genetic information Endomembrane System creates intracellular compartments with different suffices.Endoplasmic reticulum (ER rough, smooth), Golgi apparatus, lysosomes. Mitochondria generate energy to power the cell Chlorop perishs confiscate energy from sunlight, convert to carbohydrate Cytoskeleton regulates cell formulate, movements of materials within the cell, movement of the cell itself Flow of Traffic in EMS Rough ER synthesis of proteins for export (secretion) insertion into mem branes lysosomes Golgi apparatus collection, publicity & distri thoion Lysosomes * cell stomachs have enzymes that can digest * all 4 classes of biological macromolecules worn-out organelles (mitochondria replaced every 10 days) * material brought into cell by phagocytosis Phagocytosis plasma membrane engulfs smaller molecule and then called phagosome. Lysosome takes it in and digests, small particles are releases into the cytoplasm. Autophagy lysosome digests a damaged organelle, small particles are released into cytosol. mitochondria (all eukaryotic cells) and chloroplasts (plant cells) * contain DNA that encodes some ( merely not all) of their own proteins * have unusual double layers of membranesOrigin of Eukaryotic Cells Endosymbiont Theory * once believed that eukaryotes evolved gradually, organelles becoming to a greater extent and more than than complex * now accepted that early eukaryotes originated as predators * certain(p) organelles (mitochondria, chloroplasts) evo lved from smaller prokaryotes engulfed by larger cell * later chloroplasts and the skill to perform photosynthesis Symbiosis Mutual Advantage advantage to host cell * aerobic respiration (aerobic bacteria mitochondria) * photosynthesis (cyanobacteria chloroplasts) advantage to bacteria * protected environment supply of degree centigrade compounds from host cells other prey Evidence Supporting Endosymbiont Theory mitochondria and chloroplasts * are similar size to bacteria, reproduced by fission desire bacteria * have double membranes, consistent with engulfing mechanism * have their own ribosomes, which resemble those of prokaryotes rather than eukaryotes in terms of size, composition and sensitivity to antibiotics * have their own genomes, which are organized like those of bacteria last besides not least(prenominal) * are genetically similar to proposed parent bacteria rather than ukaryotic cells Cytoskeleton pregnant in * cell shape * cell motility * movement / position o f organelles * movement of materials within cell * movement of chromosomes during mitosis Cytoplasm in a living cell is never static * cytoskeleton is constantly macrocosm taken apart and rebuilt * organelles and vesicles are racing back and forth * can cross the cell in 1 atomic number 42 * unattached proteins moving randomly, but rapidly * can visit every deferral of the cell within a few seconds * contents of cytosol are in constant thermal motionCommon to all cells * selectively permeable plasma membrane * genetic code mechanism of transcription and translation * ATP for the transfer of energy and metabolic pathways Model Organisms 45 Unit 2a Intro to Cellular Chemistry Most Common Elements in Living Organisms * C H O N make up 96% also P and S are prevalent too * Exist as complex macromolecules and simpler forms like pissing and carbon dioxide nucleus dense core in centre, consists of protons and neutrons electrons continually orbit the nucleus of protons delimita te feature of an portion = atomic number protons + neutrons = mass of an atom = mass number by default, an atom is neutral, with protons = electrons electrons influence reactivity of an atom Atomic mass = atomic number + of neutrons (electrons are leave out because mass is so small) Isotopes same number of protons but different number of neutrons in the same element Anion gain electron and are negatively charged Cation lose electron and are positively chargedOutermost valence shell influences an atoms reactivity * electrons in outermost shell valence electrons * unpaired valance electrons determine the number of tie ups an atom can make * atoms with filled valance shell = most permanent, atoms that are closest to filling are most reactive * elements abundant in organisms have at least one unpaired valence electron Some Definitions covalent bonds two or more atoms contend pairs of valence electrons * strong bonds of biological systems non-covalent bonds, including * loft bonds * hydrogen bonds (H-bonds) * hydrophobic interactions olecule group of atoms held unneurotic by energy in a stable association compound molecule composed of two or more different types of atoms Types of Covalent Bonds * electrons shared equally * non-polar covalent bond * can be single (like H2), double (O2) or even triple, depending on number of electrons shared * electrons not shared equally * polar covalent bond * one of the atoms has a stronger pull on the electrons than the other * pull on electrons = electronegativity * water is the most abundant molecule in biological organisms * human body is 70% water water as a solvent can dissolve more types of molecules than other molecule known * the mansion of water is key to its role in biology hydrogen bonding electrical attraction amid disconfirming atom and partial positive of hydrogen hydrophobic no affinity for water water fearing hydrophilic affinity for water water loving hot-base Reaction substance t hat gives up (donates) protons acid (increases H+ in solution) substance that accepts protons base (decreases H+ in solution) chemical reaction that involves transfer of protons acid-base reaction * most olecules act as either an acid or a base * water can be both (both gives up and accepts protons) weak acid very few molecules dissociated (acetic acid, water) strong acid readily gives up protons (hydrochloric acid) when pH = pKa species is 50% ionized Carbon is the most distinguished element in biology carbon atoms give biomolecules their shape but other atoms attached to carbons determine their reactivity * critical H, N, O containing attachments called utilitarian groups *learn orgo working(a) groups for this courseMacromolecules * large, organized molecules that are typically created by polymerization * biological macromolecules (biomolecules) provide the structure and carry out the activities of a cell 4 groups * carbohydrates(polysaccharides) * lipids(fats) * proteins * nuc leic acids * monomers of groups are different chemical reactions used to make the gyves are similar Overview of Macromolecules 3 Proteins more functions than any other group of macromolecule * enzymes catalysis accelerate chemical reactions transport through cell membranes, in circulation * support cytoskeleton, fibres of cartilage, hair, nails * signalling / regulatory hormones, membrane proteins, intracellular messengers * movement- of the cell itself contracted proteins, flagella within the cell motor proteins * defense antibodies, complement proteins Proteins are Polymers * amino acids are connected in linear polymers of a specific date * 20 genetically encoded amino acid monomers to pick from * make of amino acids (AAs) = peptide or polypeptide polypeptide folded and coiled into a specific conformation = protein * sometimes 2 or more peptide drawing strings (subunits) combine to form mature, functional protein Amino Acid social system AAs are ionized under physi ological conditions ionization increases solubililty, facilitates interactions with to each one other and other solutes, increases reactivity (zwitterions) 7 non-ionized ionized R group unique to each AA oxygens tend to pull electrons away, fashioning it easy to lose proton gains a proton Amino Acid Side Chains R Groups * nonpolar hydrophobic R groups no charged or electronegative atoms to form H bonds * insoluble in water * R groups bury themselves with the peptide chain to hide from water * polar side chains soluble in water * uncharged but partial charges can form H-bonds * charged groups containing acids or bases highly soluble in water AA are colligate together by covalent peptide bonds carbon from carboxyl group is cogitate to N terminus of amino group. R groups and central Cs do not participate in the bond. Condensation Reaction making the chain Hydrolysis breaking the chain Polypeptide chain side chains extend from peptide-bonded backbone * chain is pliable can rotate at single bonds on either side of peptide bonds * so side chains are not all projecting to one side * chains can be from 2-3 to thousands of AAs in length * backbone is directional, convention is to number AA residues starting at N terminus this is the primary duration Sickle Cell Anemia disease in which red blood cells are abnormally shaped. Caused by single point mutation which results in substitution of single amino acid in one chain of hemoglobin protein Protein StructurePrimary Structure unique sequence of amino acids unoriginal Structure Folding into elements of structure, hydrogen bonding between amino acids(R groups not involved). 2 shapes alpha helix and important pleated sheet(parallel and antiparallel). * learn more Tertiary Structure- interactions of elements of secondary structure forming a global fold, folded into these unique shapes by ionic bonds (electrostatic),hydrogen bonds, disulphide bridges, hydrophobic interaction, van der waals dipole-dipole(al l non-covalent except for S-S). gear up of amino acids determines final shape.Maintain globular shape even if very weak. Quaternary Structure more than one polypeptide chain put together to form the final functional protein, linked by covalent and non-covalent interactions. Protein Domain segment of polypeptide that forms a compact, stable and independently folding structure. oft the building blocks for larger, more complex proteins. Disulfide bonds * covalent stabilization of protein structure found in secreted proteins (destined for a more hostile extracellular environment) * formed in ER (oxidizing environment)Once folded, do proteins ever unfold? changes in physiological or chemical conditions (pH, salt concentration, temperature) mental disturbance of H-bonds, ionic bonds, disulfide bridges, etc that maintain the proteins shape protein denatures or unfolds assertable to renature Do proteins ever fold awry(p)ly? any mutation that leads to a missing or incorrect amino aci d can lead to wrong folded protein WHY 32 Possible outcomes mutation leads to incorrectly folded protein * protein never functions properly loss of function protein folds properly at first but unfolds under certain conditions eventually loss of function * protein misfolds AND is deposited in insoluble aggregates within cell * loss of function and disruption of other aspects of cell activity * many human diseases now known to be associated with misfolded proteins . Alzheimers, cystic fibrosis, type II diabetes, retinitis pigmentosa, Parkinsons, Creutzfeldt-Jakob, some cancers *read or so catalysts and enzymes in Janelles notes, page 8-9 Nucleic Acids Information Polymers * deoxy ribo nucleic acid (DNA) sequence of subunits in DNA polymer directs RNA synthesis * ribo nucleic acid (RNA) * RNA directs ordering of AAs in a peptide chain * information stored as DNA sequences enables living organisms to pass on hereditary information * also allows each cell to pass on hereditary informa tion to the next generation of cells Monomers of Nucleic Acids Deoxyribo radicals phosphate + deoxyribose + nitrogenous base(A,C, G, or T) Ribo nucleotides phosphate + ribose + base (A,C,G, or U) Nucleic acids are linear (unbranched) polymers of nucleotides * each nucleotide consists of three parts * a nitrogenous base a (5-carbon) pentose mark * a phosphate group Purines = A&GPyramidines= C,T and U * Ribose + base = nucleoside * Ribose + base + phosphate = nucleotide Functions of Nucleotides * monomeric units of RNA and DNA * important signal molecules within cells * cyclic adenosine monophosphate (cAMP) * important agents in energy transfer reactions * cleave off phosphate group to release stored energy * act as coenzymes organic non-protein molecules required for enzyme function * usually adenine-containing nucleotides combined with B vitamins 8 condensation reaction 5 end beginning of chain. Chains always built 5 3.Look at supra case phosphate group is 5 3 end where new bases can be added polymerization rxns are endergonic * making phosphodiester bonds requires energy * energy comes from addition of 2 phosphate groups. * Activated nucleotides = nucleotide triphophates The most famous phosphorylated nucleotide adenosine triphosphate = ATP 11 adenine 4 5 5 6 1 2 3 9 4 8 7 1 3 2 O P CH2 O O O P O O O P O O O OH OH O NH2 N N N N ribose adenine + ribose (= adenosine) Secondary Structure of DNA two strands of DNA align in antiparallel arrangement with bases facing inwards. H-bonds form between bases. P P P P P P P P C C G G AA T T P O O O O O O O O O O O C G OH P Note 3 H-bonds between C and G, 2 between A and T. Only space in the starting line phosphate backbone is for Pyramidine and Purine to bond together. Features of DNA Double Helix * stabilized by H-bonds between complementary bases and hydrophobic interactions between bases * entire molecule water-soluble because charged phosphates backbone face outbound * study and minor grooves are operativ e in regulation of gene transcription Higher Order DNA Structure DNA molecules can adopt higher(prenominal) order structure Allows for compact packaging and strict regulation of gene expression RNA vs DNA like DNA sugar-phosphate backbone covalently linked by phosphodiester bonds * 4 different bases unlike DNA * uracil (U) instead of thymine (T) * pairing is A-U, C-G * sugar is ribose instead of deoxyribose * hydroxyl group makes ribose much more reactive * RNA is much less stable than DNA Secondary Structure of RNA like DNA * H-bonds form between complementary base pairs unlike DNA * most of the time, this base-pairing is between bases on the same strand * leads to formation of stem and grummet structures with single-stranded regions and double-stranded antiparallel regions * H-bonding is spontaneous, stabilizes the molecule final molecule is single-stranded * Complex folds can result in some RNA having catalytic activity Carbohydrates * Group of molecules that contain carbon, hy drogen and oxygen in a 121 ratio (CH2O)n Only monomers are in this ratio, oligomers you lose water * Monomer= monosaccharide * Dimer=disaccharide * Trimer=trisaccharide/oligosaccharide Types 1. Monosaccharides simple sugars 2. Oligosaccharides small chains (oligo=few) * Attached to proteins glycoproteins * Attached to lipids glycolipids 3. Polysaccharides very long sugar chains veritable(prenominal) Structural Features of Sugar Monomers carbonyl group (either ketone or aldehyde) * lots of -OH groups * vary in length of carbon skeleton (C3, C5, C6, ) triose, pentose, hexose * isomeric forms (glucose, fructose, galactose) * identical chemical groups arranged differently * monosaccharides often form rings in solution Isomers same atoms, different arrangements structural isomer identical groups but bonded to different carbons stereo (optical) isomer identical groups bonded to same carbons but in different orientations sixteen different hexose structures possible, all with form ula C6H12O6 C OH C OH OH H C OH H HO C H C O H C OH H H C OH H C OH H C OH H HO C H H C OH H structural isomer stereo- isomer H C C O HO C H H C OH H C OH H HO C H H C OH H fructose glucose galactose *arrangement of hydroxyl groups make a big deflexion in biological function Disaccharide 2 sugar monomer * glucose + fructose = sucrose(table sugar) * glucose + lactose = lactose * glucose + glucose = maltose makeup of disaccharides by condensation reactions. monomers are linked when C1 of one monosaccharide binds to a C on another(prenominal) often C4 geometry of bond different depending on hether OH group of C1 is in ? or ? position which C of other sugar is involved in linkage 7 C1, ? C4 ?-glucose ?-glucose maltose, ? -1,4 glycosidic bond ?-galactose ?-glucose lactose, ? -1,4 glycosidic bond (glucose has flipped over) C1, ? C4 Polymerization to build Polysaccharides amylum both are storage forms for energy starch plants glycogen animals both consist of ? -glucose monomers link ed by ? -1,4 bonds both coil into a helix (due to geometry of linkages) starch is mixture of unbranched amylose and branched amylopectin glycogen is highly branched lycogen Structural Polysaccharide in Plants Cellulose 9 polymer of ? -glucose, joined by ? -1,4 linkages each glucose is flipped relative to beside ones allows for H-bonding between adjacent strands extremely stable most abundant organic molecule on realm parallel strands joined by H-bonds Structural Polysaccharide in Animals Chitin a component of cell walls of fungi, exoskeletons of arthropods (insects, crustaceans), radulas of molluscs, beaks of cephalopods second most abundant organic molecule on earth like cellulose, joined by ? 1,4 linkages but rather than glucose, monomer is N-acetylglucosamine like cellulose, also strengthened by H-bonding btw strands 10 Structural Polysaccharide in Bacteria Peptidoglycan component of bacterial cell walls the most complex CHO so far two different change monomers linked by ? -1, 4 bonds chain of amino acids attached to one of the sugars peptide bonds instead of H-bonds (stronger) Significance of how monosaccharides are linked * ? -1-4 linkages of starch and glycogen readily hydrolyzed * ? 1-4 linkages in structural polysaccharides very resistant to enzymatic degradation For example enzymes that digest cellulose (cellulase) produced only by certain classes of bacteria, fungi and protozoa Difference between glycosidic bonds from peptide and phosphodiester bonds in common * condensation reactions different * peptide and phosphodiester bonds always occur at the same position within their monomers * each sugar monomer has several hydroxyl groups, and geometry of glycosidic bonds is highly variable Functions of Carbohydrates Structural * cellulose, chitin and peptidoglycanCell-cell recognition * membrane proteins covalently bonded to oligosaccharides Energy Storage * ? -1,4 linkages of starch and glycogen are readily hydrolyzed to release stored energy Lipids * group of carbon-containing compounds that are largely non-polar / hydrophobic * significant proportion of a given lipid molecule is hydrocarbon * the only macromolecule that is not a polymer major groups of lipids in cells * fats / oils energy storage * sterols * cholesterol membrane component * steroids hormones * * Phospholipids * major component of biological membranesFats (Triacylglycerols, Triglycerides) * form that fat is stores in apidose tissie * glycerol with 3 fat acids attached * the link between glycerol and adipose acid = ester bond condenstation rxn (liberates water) * hydrophobic * fatty acid(carboxylic acid with long hydrocarbon tail) Saturated Fatty Acid have maximum number of hydrogen atoms on each atom straight and flexible because of only single bonds Unsaturated Fatty Acid contain at least 1 double bond. The double bond is rigid and creates a kink in the chain. The rest of the chain however is free to rotate about C-C bonds.Cis H on the same side of doub le bond dont solidify easy Trans H on the opposite side of the double bond. Hydrogenation making a fat saturated/more solid at room temperature to improve shelf life therefore less healthy. Sterols group of steroids base on cholesterol(important component of cell membrane) Phospholipids * 1 glycerol, 2 fatty acids, 1 phosphate group(polar head group) * Amphipathic = hydrophilic and hydrophilic regions their most important feature with respect to biology Micelles sphere with hydrophobic tails hide in centre . Can only occur with relatively short tails Lipid Bilayer prevalent Structure for all Biological Membranes composition varies with type of organism (prokaryote vs animal vs plant vs ) type of cell within organism (muscle, liver, sperm, egg, ) type of membrane within cell (plasma membrane, Golgi, ER) inner versus outer layer different patches or domains within a particular membrane Fig 11-4 two closely apposed sheets of lipids, studded with proteins lipids serve as permea bility barrier proteins perform most of the functions carbohydrates (sugars) attached to protein and lipids in a non-random manner *all membrane lipids are amphipathic Lipid bilayers form spontaneously hydrophobic molecules would exclude water, clustering together to defame energy cost of organizing water molecules * form large droplets or surface film * amphipathic molecules are outlet to conflicting forces * solved by formation of bilayer * energetically most favourable stable, spontaneous * lipid bilayers are * closed no free edges * self-sealing * important feature for cell fusion, budding, locomotion Fluid arial mosaic Model * The plasma membrane is described to be fluid because of its hydrophobic integral components such as lipids and membrane proteins that move laterally or sideways throughout the membrane.That means the membrane is not solid, but more like a fluid. * phospholipids are constantly moving spinning in place travel laterally within leaflet * phospholipids a re occasionally flipped to the opposite leaflet during membrane synthesis but they rarely flop back * even proteins cruise slowly through the membrane Membrane liquid state how easily lipid molecules move within a membrane leaflet Alignment of phospholipid tails * tightly packed tails membrane more viscous, less fluid * freely moving tails higher fluidity What aspects of phospholipid composition influence this? length of fatty acids * from 14-24 carbons, 18-20 carbons most common * degree of saturation of fatty acids double bonds * typically one saturated fatty acid and one with one or more double bonds Cholesterol * under physiological conditions, cholesterol makes membrane stiffer less fluid * cholesterol can make up to 50% of plasma membrane lipid in some animal cells principle of Membrane Fluidity fluid state must be maintained for normal cell function strategies for maintaining membrane fluidity * change composition of membranes * alter phospholipids desaturate fatty acid s (to deal with cold) eg cold water vs sensitive water fish * change length of FA chains (yeast, bacteria) * adjust amounts of cholesterol (animals) these mechanisms have been demonstrated in * pond fish dealing with dramatic day / night temp differences * cold-resistant plants * extremophile bacteria living in hot springs * winter wheat preparing for autumn polyunsaturated FAs * sperm reduce their cholesterol just earlier fertilization Functions of Lipids * storage of chemical energy * signal molecules * vitamins * wax coating on leaves * biological membranes